trichocarpa chromosomes, and putative collinear microsyntenic regions were identified. Remarkably, a number of BAC-end pair sequences could be mapped to intervals of the sequenced Arabidopsis thaliana, V. This BAC library provides a new resource for genetic and genomic studies, as well as it represents a valuable tool for future whole genome study. We generated the first large-insert library for a member of Passifloraceae. The vast majority of the top-hits made by our sequences were to Populus trichocarpa (24.8% of the total occurrences), Theobroma cacao (21.6%), Ricinus communis (14.3%), Vitis vinifera (6.5%) and Prunus persica (3.8%). Around 9.6% of all BES were found to have high levels of similarity to plant genes and ontological terms were assigned to more than half of the sequences analysed (940). Then, we explored 10,000 BES and we identified reads likely to contain repetitive mobile elements (19.6% of all BES), simple sequence repeats and putative proteins, and to estimate the GC content (~42%) of the reads. BACs harbouring those genes were used in fluorescent in situ hybridizations and unique signals were detected for four BACs in three chromosomes ( n = 9). To check its utility for gene isolation, successful macroarray screening experiments were carried out with probes complementary to eight Passiflora gene sequences available in public databases. The library represents six haploid genome equivalents, and the average insert size was 108 kb. The library consisted of 82,944 clones and its levels of organellar DNA were very low. In this study, we have constructed a large-insert BAC library and provided the first view on the structure and content of the passion fruit genome, using BAC-end sequence (BES) data as a major resource. Therefore, improving our understanding of passion fruit genomics is essential and to some degree a pre-requisite if its genetic resources are to be used more efficiently.
However, very little is known about the genome of the species. The juice is also used in concentrate blends that are consumed worldwide.
The passion fruit ( Passiflora edulis) is a tropical crop of economic importance both for juice production and consumption as fresh fruit.
0 kommentar(er)
